Plasmid

Part:BBa_K1391016:Design

Designed by: Jing Wei "Raymond" Liu   Group: iGEM14_MIT   (2014-10-17)


pENTR_hEF1a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1211
    Illegal PstI site found at 338
    Illegal PstI site found at 843
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1211
    Illegal PstI site found at 338
    Illegal PstI site found at 843
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1211
    Illegal BglII site found at 592
    Illegal BamHI site found at 1198
    Illegal XhoI site found at 991
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1211
    Illegal PstI site found at 338
    Illegal PstI site found at 843
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1211
    Illegal PstI site found at 338
    Illegal PstI site found at 843
    Illegal NgoMIV site found at 726
    Illegal AgeI site found at 104
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

human

References